NAD+ Reconstitution Guidelines

Concentration and Volume Recommendations

Reconstitute a 1000 mg NAD⁺ vial with 10 mL bacteriostatic water to obtain a 100 mg/mL solution, providing dosing flexibility and good stability. 1
For a 50 mg/mL concentration, add 20 mL bacteriostatic water. 1
For a 200 mg/mL concentration, add 5 mL bacteriostatic water; higher concentrations reduce injection volume but may compromise stability. [1][2]
The lyophilized powder contributes roughly 1 mL to the final volume, yielding an approximate total volume of 11 mL after reconstitution. 3

Sanitize the vial stopper with 70 % isopropanol before needle insertion to prevent microbial contamination. 4
Maintain sterile technique throughout the process, avoiding direct contact of the needle, syringe, or stopper with non‑sterile surfaces. 4
Inject the diluent slowly down the side of the vial to minimize foaming and preserve solution integrity. 4
Gently swirl the vial (do not shake vigorously) until the powder is fully dissolved. 4
Perform a visual inspection after mixing, checking for clarity, absence of particulate matter, and overall physical integrity. 4

Label the reconstituted vial with patient identifier, concentration, reconstitution date, and beyond‑use date. 4
Bacteriostatic water contains a preservative (≈ 0.9 % benzyl alcohol) that permits multi‑dose use for up to 28 days when stored refrigerated. 4
Store the reconstituted NAD⁺ solution at 2 – 8 °C (36 – 46 °F). 2

Do not retain reconstituted solutions long‑term unless specific stability data support extended use; many compounds become unstable after reconstitution. [3][2]
Discard the solution if it becomes discolored or contains particulate matter. 2
Use the reconstituted product within the recommended timeframe; assume limited stability in the absence of validated data. [3][2]

Never use sterile water for injection without preservative for multi‑dose vials, as this raises the risk of contamination. 4
Avoid over‑dilution, which can jeopardize stability and necessitate larger injection volumes. 2
Do not reconstitute powder that has been exposed to moisture or appears discolored prior to mixing. 2