ELISA Applications and Procedures

Clinical Applications

The American Journal of Transplantation recommends the use of ELISA for evaluating candidates for renal transplantation to detect anti-HLA antibodies 1
ELISA can be used for the diagnosis of infectious diseases, such as detecting antibodies against specific pathogens or antigens, as recommended by Critical Care 2
The detection of antibodies against Helicobacter pylori can be done using ELISA, as stated in Clinical Microbiology and Infection 3
ELISA can be used to diagnose autoimmune diseases, such as pemphigoid, by detecting antibodies against BP180 and BP230, as recommended by the British Journal of Dermatology 4
The detection of invasive candidiasis can be done using ELISA by detecting antigen manano (Mn) and anti-manano antibodies (A-Mn), as stated in Critical Care 2, 5
ELISA can be used to diagnose fungal infections, such as detecting galactomannan for the diagnosis of invasive aspergillosis, as recommended by Annals of Oncology 7
The diagnosis of Q fever can be done using ELISA by detecting antibodies against Coxiella burnetii, as stated in MMWR Recommendations and Reports 8
ELISA can be used for the analysis of bioaerosols for epidemiological studies and environmental risk assessment, as recommended by the Journal of Environmental Sciences (China) 9, 10

The procedure of ELISA includes blocking to prevent non-specific bindings, as recommended by the American Journal of Transplantation 1
The results of ELISA should be interpreted in the context of clinical history and other laboratory findings, as stated in Clinical Microbiology and Infection and British Journal of Dermatology 3, 4
ELISA has limitations, such as possible cross-reactions between related species, as recommended by MMWR Recommendations and Reports 8
The standardization of ELISA protocols is necessary to ensure reliable results, as stated in the Journal of Environmental Sciences (China) 9, 10
The control of quality is crucial in ELISA, including the use of positive and negative controls, as recommended by the Journal of Environmental Sciences (China) 10