Embryo Blastocyst Grading in IVF

Optimal Biopsy Technique

Blastocyst trophoblast cell biopsy is the main recommended method for embryo assessment in IVF cycles, as it has minimal effect on embryo development potential 1
Biopsy should be performed on Day 5 or 6 after ICSI when the blastocyst is fully expanded 1
The biopsy should be performed away from the inner cell mass to avoid damage to this critical structure 1
Five to eight trophectoderm cells should be biopsied - this number balances the need for sufficient genetic material while minimizing impact on embryonic development 1, 2

Single embryo transfer is recommended for patients with PGT-M to reduce multiple pregnancy risks 3

Embryo grading should be performed by trained embryologists with regular quality control assessments 3