Praxis Medical Insights

Est. 2024 • Clinical Guidelines Distilled

Made possible by volunteer editors from the University of Calgary & University of Alberta

Last Updated: 12/2/2025

Gynecological Examination Guidelines for Reproductive-Age Women

Essential Instruments and Their Specific Uses

  • The Centers for Disease Control and Prevention recommends using a speculum, cytobrush or endocervical brush for Pap testing, and Ayre's spatula for ectocervical sampling, with adequate lubrication to minimize discomfort in reproductive-age women 1
  • The American College of Obstetricians and Gynecologists suggests that cytobrush is the preferred instrument for endocervical sampling as it improves the number of satisfactory Pap tests 1
  • Ayre's spatula should be used for ectocervical sampling to ensure adequate squamous cell collection in reproductive-age women 1
  • Broom devices are acceptable alternatives and also improve satisfactory test results in reproductive-age women 1
  • It is important to note that cytobrushes should not be used in pregnant women, and only a swab and Ayre's spatula should be used during pregnancy 1

Specimen Collection and Critical Technique Considerations

  • Cotton swabs (saline-soaked) should be used for removing mucopurulent discharge before Pap testing when present in reproductive-age women 1
  • Sterile swabs should be used for collecting specimens for sexually transmitted infection testing (gonorrhea, chlamydia) in reproductive-age women 1
  • The sequence of Pap testing in relation to other cervicovaginal specimen collection does not influence results, so collect specimens in whatever order is most efficient in reproductive-age women 1
  • If mucopurulent discharge is present, it should be carefully removed with a saline-soaked cotton swab before performing the Pap test in reproductive-age women 1

Special Considerations for Pap Testing

  • If the patient is menstruating, conventional cytology Pap testing should be postponed and rescheduled at the earliest opportunity 1
  • The presence of infection does not require delaying the Pap test in most instances, as the test will still be satisfactory for evaluation in reproductive-age women 1
  • If a Pap test is reported as unsatisfactory, it must be repeated and determined to be satisfactory and negative before resuming regular screening intervals in reproductive-age women 1
  • Cytobrushes should never be used in pregnant patients, as this can cause bleeding and patient discomfort 1

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