Molecular Diagnostic Techniques for Candida auris Detection

Limitations and Guidelines

• The European Society of Clinical Microbiology and Infectious Diseases (ESCMID) guidelines note that molecular detection techniques for Candida species, including Candida auris, lack external validation and standardization, with a need for third-party appraisal of results and harmonization of PCR-based techniques before definitive recommendations can be made regarding clinical utility 1, 2, 3
• In-house PCR techniques for Candida detection have shown pooled sensitivity and specificity over 85% in meta-analyses, but the variability in materials and methods used across different laboratories remains a challenge 4, 5