Platelet-Rich Plasma Preparation and Clinical Applications

Preparation Methodology

Single-spin PRP uses one low-force centrifugation step to separate platelet-rich plasma from whole blood, resulting in a relatively pure preparation with good yield, though platelet enrichment is limited 1, 2
Double-spin PRP involves two sequential centrifugation steps to concentrate platelets further, achieving higher platelet concentrations and better clinical outcomes 3, 4, 5
The presence of leukocytes in PRP may impact biology through immune and antibacterial properties, as well as tissue healing and local growth factor regulation 1, 2
Single-spin methods typically retain more white blood cells (10^8 to 10^9 WBCs per unit) 7

Blood should be collected in plastic tubes using trisodium citrate, ACD, or CPD anticoagulants, and processed at room temperature as soon as possible, preferably within 1 hour of collection 1, 2, 8
Freshly prepared PRP should be used within 4 hours for optimal results 8
Activation protocols should include calcium chloride, autologous thrombin, or collagen type I, depending on the clinical application 8

Do not use EDTA as an anticoagulant, as it causes premature platelet activation 1, 2, 8
Avoid storing PRP at 4°C, as cold activation may occur prematurely 8
Ensure proper documentation of the specific preparation method used, as this significantly impacts reproducibility and clinical outcomes 1, 2