Cord Blood Typing and Transplantation Guidelines

HLA Typing and Matching for Transplantation

• Cord blood units must be typed by DNA-based methods for HLA-A and -B at minimum antigen-level resolution and for DRB1 at high resolution, with HLA-C typing strongly recommended 1
• High-resolution typing for all loci helps characterize potential allele-level mismatches 1
• For transplantation, ≥4/6 loci should be matched for malignant hematological diseases 2

Transplantation Recommendations

• The American Society of Transplantation recommends using flow cytometry or complement-dependent cytotoxicity assay for all patients 2
• A positive crossmatch against T cell or B cell donor targets is a contraindication to transplantation 2
• Total nucleated cell count requirements: >2.5-4.0 × 10^7/kg 2