Stool Sample Handling for Fecal Calprotectin Testing

Guideline Recommendations

• The British Society of Gastroenterology advises that stool samples for fecal calprotectin should be stored at room temperature for no longer than 3 days before analysis, emphasizing the need for timely processing to avoid degradation. 1

Sample Collection Timing

• Collecting the first‑morning stool is recommended because it reduces day‑to‑day variability in calprotectin concentrations, providing a more reliable baseline for assessment. 2

Sample Preservation Practices

• Avoid freeze‑thaw cycles when handling stool specimens; freezing and subsequent thawing can compromise calprotectin stability and is not recommended for routine testing. 3

Temperature‑Controlled Storage and Timely Processing of Faecal Calprotectin Samples

Recommended Storage Conditions and Processing Window

• Storing stool specimens for faecal calprotectin at ≤ 4 °C and analyzing them within 24 hours preserves calprotectin stability and prevents degradation, as recommended by the British Society of Gastroenterology 4 (evidence level not specified).