Praxis Medical Insights

Est. 2024 • Clinical Guidelines Distilled

Made possible by volunteer editors from the University of Calgary & University of Alberta

Last Updated: 9/23/2025

Identifying the L2 Vertebra

Primary Landmark Method

  • The iliac crests serve as the most reliable surface landmark, typically aligning with the L4-L5 intervertebral disc space, and from this reference point, counting upward identifies L2 1, 2, 3
  • The "bottom-up" counting method is the standard approach recommended for DXA imaging and clinical practice, using the iliac crests as the primary landmark 1, 2, 3

Alternative Anatomical Landmarks

  • The conus medullaris normally ends at the L1-L2 disc space, which can be useful for identifying L2 on MRI 4

Critical Pitfalls to Avoid

  • Anatomical variants with 4 or 6 lumbar vertebrae can occur and should be recognized to avoid miscounting 1, 2, 3
  • If specific vertebral labeling verification is needed, obtaining a full spine radiograph, CT, or MRI can confirm the exact level 1, 2, 3

Imaging Confirmation

  • On radiographs or CT, counting from the sacrum upward and confirming L5 as the vertebra articulating with the sacrum can identify L2, which will be three vertebral bodies above L5 1, 2
  • On MRI, the conus medullaris can be used as an additional reference to identify L2, and iliac crest alignment at L4-L5 can be confirmed 1, 2, 4

REFERENCES

1

updated practice guideline for dual-energy x-ray absorptiometry (dxa). [LINK]

European Journal of Nuclear Medicine and Molecular Imaging, 2025

2

updated practice guideline for dual-energy x-ray absorptiometry (dxa). [LINK]

European Journal of Nuclear Medicine and Molecular Imaging, 2025

3

updated practice guideline for dual-energy x-ray absorptiometry (dxa). [LINK]

European Journal of Nuclear Medicine and Molecular Imaging, 2025